LP115359-4     Chlamydia trachomatis and Neisseria gonorrhoeae

DESCRIPTION
  Part: Chlamydia trachomatis & Neisseria gonorrhoeae
  Description: Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. The GEN-PROBE APTIMA Combo 2 Assay replicates a specific region of the 23S rRNA from CT and a specific region of the 16S rRNA from GC via DNA intermediates. A unique set of primers is used for each target molecule. Detection of the rRNA amplification product sequences (amplicon) is achieved using nucleic acid hybridization. Single-stranded chemiluminescent DNA probes, which are complementary to a region of each target amplicon, are labeled with different acridinium ester molecules. The labeled DNA probes combine with amplicon to form stable RNA:DNA hybrids. The Selection Reagent differentiates hybridized from unhybridized probe, eliminating the generation of signal from unhybridized probe. During the detection step, light emitted from the labeled RNA:DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). In DKA, differences in the kinetic profiles of the CT and GC labeled probes allow for the differentiation of signal; kinetic profiles are derived from measurements of photon output during the detection read time. The chemiluminescent detection reaction for CT signal has very rapid kinetics and has the “flasher” kinetic type. The chemiluminescent detection reaction for GC signal is relatively slower and has the “glower” kinetic type. Assay results are determined by a cut-off based on the total RLU and the kinetic curve type.
 
 

BASIC PART PROPERTIES
  Part: Chlamydia trachomatis & Neisseria gonorrhoeae
  Construct for LOINC short name: C trach+GC
  Type: COMPONENT
  Status: ACTIVE
  Created On: 3/22/2011

LANGUAGE VARIANTS
 
  Chinese (CHINA)  (From: Lin Zhang, A LOINC volunteer from China)   SYNONYMS
  沙眼衣原体与淋病奈瑟菌     沙眼衣原体与淋病奈瑟菌(淋球菌、淋病、淋病双球菌、淋病奈瑟氏菌)
 
  Dutch (NETHERLANDS)  (From: NVKC, Dutch Society for Clinical Chemistry and Laboratory Medicine, The Netherlands)    
  Chlamydia trachomatis & Neisseria gonorrhoeae      
 
  French (CANADA)  (From: Canada Health Infoway Inc.)    
  Chlamydia trachomatis & Neisseria gonorrhoeae      
 
  Italian (ITALY)  (From: Consiglio Nazionale delle Ricerche)   SYNONYMS
  Chlamydia trachomatis & Neisseria gonorrhoeae     Chlamydia trachomatis e Neisseria gonorrhoeae
 
  Russian (RUSSIAN FEDERATION)  (From: Yaroslavl State Medical Academy)    
  Chlamydia trachomatis & Neisseria gonorrhoeae      
 
  Spanish (SPAIN)  (From: the Clinical Laboratory Committee of SERVICIO EXTREMEÑO DE SALUD, with the support of BITAC MAP.)   SYNONYMS
  Chlamydia trachomatis & Neisseria gonorrhoeae     Chlamydia trachomatis y Neisseria gonorrhoeae

INTERNAL FIELDS
  Detail Page Created On: 6/20/2019 9:23:10 AM
  Display name: Chlamydia trachomatis and Neisseria gonorrhoeae
  Preferred Abbreviation: C trach+GC