Version 2.77

Description

Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. The GEN-PROBE APTIMA Combo 2 Assay replicates a specific region of the 23S rRNA from CT and a specific region of the 16S rRNA from GC via DNA intermediates. A unique set of primers is used for each target molecule. Detection of the rRNA amplification product sequences (amplicon) is achieved using nucleic acid hybridization. Single-stranded chemiluminescent DNA probes, which are complementary to a region of each target amplicon, are labeled with different acridinium ester molecules. The labeled DNA probes combine with amplicon to form stable RNA:DNA hybrids. The Selection Reagent differentiates hybridized from unhybridized probe, eliminating the generation of signal from unhybridized probe. During the detection step, light emitted from the labeled RNA:DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). In DKA, differences in the kinetic profiles of the CT and GC labeled probes allow for the differentiation of signal; kinetic profiles are derived from measurements of photon output during the detection read time. The chemiluminescent detection reaction for CT signal has very rapid kinetics and has the "flasher" kinetic type. The chemiluminescent detection reaction for GC signal is relatively slower and has the "glower" kinetic type. Assay results are determined by a cut-off based on the total RLU and the kinetic curve type. Source: Regenstrief Help

Basic Part Properties

Part Display Name
Chlamydia trachomatis and Neisseria gonorrhoeae rRNA panel
Part Type
Component (Describes the core component or analyte measured)
Created On
2011-03-23
Construct for LOINC Short Name
C trach+GC pnl

LOINC Terminology Service (API) using HL7® FHIR® Get Info

CodeSystem lookup
https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=LP115366-9

Language Variants Get Info

Tag Language Translation
zh-CN Chinese (China) 沙眼衣原体与淋病奈瑟菌 rRNA 组套
Synonyms: 沙眼衣原体与淋病奈瑟菌(淋球菌、淋病、淋病双球菌、淋病奈瑟氏菌) rRNA 组套(组合、医嘱组、套餐、套餐医嘱、医嘱套餐、组合申请、组合项目);沙眼衣原体与淋病奈瑟菌 rRNA 组套;沙眼衣原体与淋病奈瑟菌 rRNA 组合;沙眼衣原体与淋病奈瑟菌核糖体核糖核酸医嘱组
fr-CA French (Canada) Chlamydia trachomatis & Neisseria gonorrhoeae, ARNr
es-ES Spanish (Spain) Perfil de rRNA de Chlamydia trachomatis y Neisseria gonorrhoeae
it-IT Italian (Italy) Chlamydia trachomatis & Neisseria gonorrhoeae, panel rRNA
Synonyms: Panel rRNA Chlamydia trachomatis e Neisseria gonor
tr-TR Turkish (Turkey) Chlamydia trachomatis ve Neisseria gonorrhoeae rRNA paneli
ru-RU Russian (Russian Federation) Chlamydia trachomatis & Neisseria gonorrhoeae рРНК панель
nl-NL Dutch (Netherlands) Chlamydia trachomatis & Neisseria gonorrhoeae rRNA panel