Version 2.77

Term Description

This assay detects the presence of one or more of the Escherichia coli enterotoxigenic ltA, st1a and st1b toxins using PCR and melt curve analysis. The assay does not distinguish between the three toxins. This term was created for, but is not limited in use to, BioFire's FilmArray Gastrointestinal panel, which is intended for the detection and identification of nucleic acids from multiple bacteria, viruses, and parasites directly from stool samples from individuals with signs and/or symptoms of gastrointestinal infection.
Source: Regenstrief LOINC

Part Description

LP200472-1   Escherichia coli enterotoxigenic ltA+st1a+st1b genes
Enterotoxigenic Escherichia coli (ETEC) produces a heat-labile (LT) and a heat-stable (ST) enterotoxin, both of which cause severe diarrhea in both humans and animals. ST is an extracellular toxin which is comprised of a methanol-soluble (STa) toxin and a methanol-insoluble (STb) toxin. The sta gene encodes the STa toxin and the stb gene encodes the STb toxin. [UniProt: Q70Q79]; [UniProt: Q70Q73] STa combines to its enterocyte receptor, guanylate cyclase, which causes intestinal secretion and diarrhea. PMID: 8528070 The STb enterocyte receptor is the protein sulfatide. After STb binds to sulfatide, it is engulfed by the enterocyte. It then activates the pertussis toxin-sensitive G protein which opens the ligand-gated calcium ion channel, allowing an influx of calcium ions. A subsequent secretion of chloride ions, stimulation of arachidonic acid release, and production of prostaglandin E2 and 5-dyroxytryptamine leads to an efflux of water, bicarbonate ions, sodium ions, and chloride ions into the intestinal space, all of which leads to watery diarrhea. PMID: 23716609 Source: Regenstrief LOINC

Fully-Specified Name

Component
Escherichia coli enterotoxigenic ltA+st1a+st1b genes
Property
PrThr
Time
Pt
System
Stool
Scale
Ord
Method
Non-probe.amp.tar

Additional Names

Short Name
ETEC ltA+st1a+st1b tox St NAA+non-probe
Display Name
E. coli enterotoxigenic ltA+st1a+st1b genes NAA+non-probe Ql (Stl)
Consumer Name Alpha Get Info
Escherichia coli enterotoxigenic ltA+st1a+st1b genes, Stool

Example Answer List: LL744-4

Source: Regenstrief Institute
Answer Code Score Answer ID
Detected LA11882-0
Not detected LA11883-8

Basic Attributes

Class
MICRO
Type
Laboratory
First Released
Version 2.54
Last Updated
Version 2.73
Change Reason
Changed component name to clarify that this assay detects the toxin genes (vs. protein). Changed Method from "Melt.amp.tar" to "Non-probe.amp.tar", which includes other non-probe based detection methods while still distinguishing from probe-based methods.; The PrThr property is used for LOINC terms whose results are reported using an ordered categorical scale, regardless of whether or not an internal threshold was used to make that determination. This change was approved by the Laboratory LOINC Committee in June 2016.
Order vs. Observation
Observation
Common Test Rank Get Info
1353

Member of these Panels

LOINC Long Common Name
82195-9 Gastrointestinal pathogens DNA and RNA panel - Stool by NAA with non-probe detection

Language Variants Get Info

Tag Language Translation
de-DE German (Germany) Escherichia coli enterotoxigen ltA+st1a+st1b-Gene:Nachweis oder Schwellenwert:Zeitpunkt:Stuhl:Ordinal:Zielfragmentamplifikation mit Detektion ohne Sonde
es-ES Spanish (Spain) toxinas ltA+st1a+st1b de Escherichia coli enterotoxigénica:PrThr:Punto temporal:Heces:Ord:Amplificación del objetivo no basado en sonda
es-MX Spanish (Mexico) Genes enterotoxigénicos ltA + st1a + st1b de Escherichia coli:Presencia o umbral:Punto temporal:Heces:Ordinal:Amplificación de ácidos nucleicos con detección sin sonda
fr-FR French (France) Escherichia coli entérotoxinogène ltA+st1a+st1b gènes:Présence/Seuil:Ponctuel:Selle:Qualitatif:Non-PCR amplification de cible
it-IT Italian (Italy) Escherichia coli enterotossigeni geni ItA+st1a+st1b:PrThr:Pt:Feci:Ord:Non-sonda.amp.tar
Synonyms: Amplificazione target non basata su sonda ETEC Microbiologia Presenza o Soglia Punto nel tempo (episodio) Sonda con amplificazione Sonda con amplificazione del target Sonda di DNA
zh-CN Chinese (China) 大肠埃希菌肠产毒素型 ltA+st1a+st1b 基因:存在情况或阈值:时间点:大便:序数型:非探针法.基因扩增.靶向
Synonyms: E coli;大肠埃希氏杆菌;大肠埃希氏菌;大肠杆菌 产肠毒素性大肠埃希菌（E coli、大肠埃希氏杆菌、大肠埃希氏菌、大肠杆菌）;肠毒性大肠杆菌;肠产毒性大肠杆菌;ETEC 依次型;分类顺序型;定性的;序数型（或称等级型）;性质上的;有序型;有序性分类应答;有序性分类结果;秩次型;等级型;筛查;顺序型 便;大肠运动;排便;粪便;肠运动 借助扩增的探针方法;借助扩增的探针法;探针法.扩增 借助靶向扩增的探针方法;借助靶向扩增的探针法;探针法.扩增.靶向 借助靶向扩增的非探针方法;借助靶向扩增的非探针法;非探针法.扩增.靶向 分子病理学 - 基因类 基因 大肠埃希菌（E coli、大肠埃希氏杆菌、大肠埃希氏菌、大肠杆菌）肠产毒素型（肠产毒素性、产肠毒素性、产肠毒素型、肠毒性、肠毒型、产肠毒性、产肠毒型） ltA+st1a+st1b 基因;肠产毒素型大肠埃希菌 ltA+st1a+st1b 基因 存在情况;存在;存在与否;是否存在;阈值;界值;界限;阀值;临界值;存在情况（存在、存在与否、是否存在）或阈值（界值、界限、阀值、临界值） 微生物学;微生物学试验;微生物学试验（培养、DNA、抗原及抗体） 扩增型 扩增性 时刻;随机;随意;瞬间 经过扩增的 脱氧核糖核酸探针

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CodeSystem lookup
https://fhir.loinc.org/CodeSystem/$lookup?system=http://loinc.org&code=80351-0